This web site, https://www.vcru.wisc.edu/sdata, contains data from the laboratory of Philipp W. Simon, USDA-ARS Vegetable Crops Research Unit [Click here for our web page]
|
USDA ARS VCRU Data Server This web site, https://www.vcru.wisc.edu/sdata, contains data from the laboratory of Philipp W. Simon, USDA-ARS Vegetable Crops Research Unit [Click here for our web page] |
Link to Population MP2
Demonstration of linkage and developement of the first low-density genetic map of garlic, based on AFLP markers. M. Ipek, A. Ipek, S.G. Almquist, P.W. Simon. Theor Appl Genet (2005) 110: 228-236
Fig.1 Linkage groups in the genetic map of Allium sativum based on the MP1 family. Map distances are in cM. Bolded numbers are common markers segregating in both MP1 and MP2.
Table 1 The number AFLP markers scored in each primer combination and their segregation ratios. Numbers within the parentheses are the number of markers mapped in either major or minor linkage groups.
MP1 family
| Primer combination | AFLP markers | ||||
| EcoR I | Mse I | Total | 3:1 | 15:1 | 1:1 |
| AAGA | CAA | 11 | 8(3) | 1 | 2 |
| CAC | 12 | 9(7) | 2 | 1 | |
| CAG | 11 | 8(6) | 2 | 1 | |
| CCA | 11 | 11(7) | - | - | |
| CCG | 21 | 20(14) | 1 | - | |
| CCT | 11 | 8(5) | 3 | - | |
| CGC | 14 | 13(7) | 1 | - | |
| CGG | 24 | 23(19) | 1 | - | |
| CGT | 30 | 26(24) | 3 | 1 | |
| CTA | 1 | 1(1) | - | - | |
| AAGG | CAA | 11 | 10(6) | 1 | - |
| CAG | 16 | 10(8) | 6 | - | |
| CCT | 18 | 18(16) | - | - | |
| CTG | 10 | 9(7) | 1 | - | |
| ACGA | CAA | 10 | 9(7) | 1 | - |
| CAC | 14 | 13(6) | 1 | - | |
| CAG | 8 | 7(5) | 1 | - | |
| CAT | 13 | 10(6) | 2 | 1 | |
| CCT | 21 | 18(14) | 3 | - | |
| CTG | 11 | 8(6) | 3 | - | |
| ACGG | CAC | 14 | 12(8) | 2 | - |
| CAG | 12 | 10(7) | 2 | - | |
| CTC | 7 | 5(1) | 2 | - | |
| CATT | 11 | 9(5) | 2 | - | |
| AGCT | CAA | 11 | 10(7) | 1 | - |
| CAC | 16 | 10(5) | 6 | - | |
| ATGA | CAG | 6 | 6(4) | - | - |
| CAT | 5 | 4(1) | 1 | - | |
| Total | 360 | 305(212) | 49 | 6 | |
Table 2 Oligo nucleotide sequences of gene specific primers based on the genomic sequences of garlic.
| Gene | Primer set | Sequence (5’-3’) |
| Alliinase | As-Alliinase-1 As-Alliinase-2 |
CTCAACTCATCCATGGACTCGTCATCTCT GATCGTACGTTAGATCGATGTGTGC |
| SST-1 | As-SST1-1 As-SST1-2 |
TGGACAATGATGAGTACATGTCAGTCGC CAGATAATTTTGATTACAGAGAATTTGCTGTCAACTT |
| Chitinase | As-Chtn-1 As-Chtn-2 |
CAGCAACAGGCTATGCTGTAGC GAATGAGTTTGCAGCTGCTATGAAGG |
| CHS | As-CHS-1 As-CHS-2 |
GTGAAGCGCTTCATGATGTACCA GGATGCGCTATCCAAAACACCT |
Table 3 Marker distribution among the linkage groups of garlic in two families.
MP1 family
| Linkage group |
Marker Number |
Length (cM) |
Average Distance (cM) |
| MP1-i | 21 | 100 | 4.7 |
| MP1-ii | 18 | 85 | 4.7 |
| MP1-iii | 14 | 59 | 4.2 |
| MP1-iv | 13 | 45 | 3.4 |
| MP1-v | 12 | 67 | 5.5 |
| MP1-vi | 11 | 60 | 5.4 |
| MP1-vii | 11 | 60 | 5.4 |
| MP1-viii | 10 | 70 | 7.0 |
| MP1-ix | 10 | 49 | 4.9 |
| MP1-x | 8 | 30 | 3.7 |
| MP1-xi | 7 | 57 | 8.1 |
| MP1-xii | 7 | 21 | 3.0 |
| MP1-xiii | 6 | 34 | 5.6 |
| MP1-xiv | 5 | 35 | 7.0 |
| MP1-xv | 5 | 34 | 6.8 |
| MP1-xvi | 5 | 33 | 6.6 |
| MP1-xvii | 5 | 31 | 6.2 |
| MP1-xviii | 5 | 24 | 4.8 |
| MP1-xix | 5 | 21 | 4.2 |
| Others | 38 | 251 | 6.6 |
| Total | 216 | 1166 | 5.4 |
