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Brasilia 1252×B6274 AFLP Linkage Map

This map was published in Characterization of the Meloidogyne javanica resistance locus employing molecular markers and isolation of candidate disease resistance loci in the carrot (Daucus carota L.) genome
by Leonardo Silva Boiteux, Ph.D. Thesis, 2000, University of Wisconsin-Madison
Chapter 6: Molecular Tagging of the Meloidogyne javanica Resistance Locus with Amplified Fragment Length Polymorphism (AFLP) Markers and Sequence Characterization of AFLP Loci in Carrot (Daucus carota)

Details about the markers

Details about individual markers are available by clicking on any of the markers on the map, or by going directly to the marker information page

Linkage map

Linkage relationships between the Mj-1 locus and AFLP and AFLP-derived markers were estimated using MAPMAKER version 3.0 for IBM-PC (Lander et al., 1987; Lincoln et al. 1992). Linkage groups were first assigned based upon two-point analysis via ‘group’ command. Markers were initially associated with two-point comparisons of the group command (threshold LOD score = 5.0 and recombination fraction 0.25). The most likely order of the markers was obtained using exhaustive multipoint analysis via ‘compare’ command and the final maximum likelihood map was obtained with ‘map’ command. Recombination frequencies were transformed to Kosambi map distances (Kosambi, 1944).

Figure 6.2. The AFLP linkage map of the Mj-1 (Meloidogyne javanica resistance) locus in carrot. The map population was composed by 188 F₂ individuals from the cross ‘Brasilia-1252’ (resistant parent) × ‘6274B’ (susceptible parent). The genetic distance in cM (Kosambi function) between pairs of markers is shown at the left and the AFLP marker designation is shown on the right

P7B9Z P5B6Z P4B5Z P6B6U Mj-1 P3B3Z P8B8U P4B2U P8B3Z P6B11U P5B11U