This web site, https://www.vcru.wisc.edu/sdata, contains data from the laboratory of Philipp W. Simon, USDA-ARS Vegetable Crops Research Unit [Click here for our web page]
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USDA ARS VCRU Data Server This web site, https://www.vcru.wisc.edu/sdata, contains data from the laboratory of Philipp W. Simon, USDA-ARS Vegetable Crops Research Unit [Click here for our web page] |
Expression and mapping of anthocyanin biosynthesis genes in carrot
Mehtap Yildiz, David K. Willis, Pablo F. Cavagnaro, Massimo Iorizzo, Kazim Abak, Philipp W. Simon
Theoretical and Applied Genetics
July 2013, Volume 126, Issue 7, pp 1689-1702 doi:10.1007/s00122-013-2084-y
Anthocyanin gene expression has been extensively studied in leaves, fruits and flowers of numerous plants. Little, however, is known about anthocyanin accumulation in roots of carrots or other species. We quantified expression of six anthocyanin biosynthetic genes [phenylalanine ammonia-lyase (PAL3), chalcone synthase (CHS1), flavanone 3-hydroxylase (F3H), dihydroflavonol 4-reductase (DFR1), leucoanthocyanidin dioxygenase (LDOX2), and UDP-glucose:flavonoid 3-O-glucosyltransferase (UFGT)] in three carrot inbreds with contrasting root color: solid purple (phloem and xylem); purple outer phloem/orange xylem; and orange phloem and xylem. Transcripts for five of these genes (CHS1, DFR1, F3H, LDOX2, PAL3) accumulated at high levels in solid purple carrots, less in purple-orange carrot, and low or no transcript in orange carrots. Gene expression coincided with anthocyanin accumulation. In contrast, UFGT expression was comparable in purple and orange carrots and relatively unchanged during root development. In addition, five anthocyanin biosynthesis genes [FLS1 (flavonol synthase), F3H, LDOX2, PAL3, and UFGT] and three anthocyanin transcription factors (DcEFR1, DcMYB3 and DcMYB5) were mapped in a population segregating for the P1 locus that conditions purple root color. P1 mapped to chromosome 3 and of the eight anthocyanin biosynthesis genes, only F3H and FLS1 were linked to P1. The gene expression and mapping data suggest a coordinated regulatory control of anthocyanin expression in carrot root and establish a framework for studying the anthocyanin pathway in carrots, and they also suggest that none of the genes evaluated is a candidate for P1.
From Brian Just Ph.D. Thesis:
page 100
Population 10117 was developed by crossing two unreleased USDA inbreds.
One parent was a true-breeding yellow-rooted F4M inbred derived from the cross
PI173687 × B493.
The other parent was a true breeding purple/orange-rooted F5M inbred from the cross
PI173687 × B10138.
This parent had a purple exterior and an orange interior.
The F1 hybrid had a purple exterior and a yellow interior.
A single F1 hybrid was self-pollinated to produce population 10117.
Figure 4.2 shows all roots from this population.
The F2 generation used in this study was grown on Paul Miller Farms, Hancock, WI under
commercial growing conditions in the summer of 2001.
Roots were harvested and had tissue sampled for DNA extraction in September 2001.
The same population was grown again in the summer of 2002.
Roots were stored at 4°C for approximately 2.5 months and were re-planted in the greenhouse in
December 2002 for self-pollination.
The seed packet for this population is 44234
Thesis Figure 4.2 - Click to enlarge
Marker DcEFR1 should instead be named DcERF1 both on the map and in the publication
Marker FLS1 is shown as FLS on the map
Genetic linkage maps of chromosomes of the 10117 carrot population that contain anthocyanin structural and regulatory genes. A and B refer to the purple-orange- ("A") and yellow- ("B") rooted parents. Anthocyanin genes and microsatellites are denoted in red and blue letters, respectively. P1 (Chromosome 3) and Y2 (Chromosome 7) are phenotypic loci denoted in purple and orange letters, respectively. Codominant markers present in both parent maps are underlined. LGs were oriented and named according to their corresponding physical chromosomes (Iovene et al. 2011).
| Marker info | Left=QAL Right=B493 | Marker info |
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